Globins and Other Nitric Oxide-Reactive Proteins, Part A

Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years' experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.

Section I. Nitric Oxide: Chemical and Analytical Methods

Chapter 1
The Chemistry of Nitric Oxide and Related Species
Martin H. Hughes

Chapter 2
Delivery of nitric oxide for analysis of the function of cytochrome c'.
Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir

Chapter 3
The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems
Rubina G. Aga and Martin N. Hughes

Chapter 4
The Chemistry of Peroxynitrite - Implications for Biological Activity
Sara Goldstein and Gabor Mernyi

Chapter 5
Nitric Oxide (NO) Selective Electrodes
Ian R. Davies, Xueji Zhang

Chapter 6
NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode
L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries

Chapter 7
Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples
Jay R. Laver, Tnia M. Stevanin, Robert C. Read

Section II. Bacterial and Archaeal Hemoglobins

Chapter 8
Interactions of nitric oxide with hemoglobin: from microbes to man
Michael Angelo, Alfred Hausladen and Jonathan S. Stamler

Chapter 9
Expression and purification of E. coli Hmp
Robert K. Poole

Chapter 10
Structural studies on flavohemoglobins
Andrea Ilari and Alberto Boffi

Chapter 11
Flavohemoglobin of Staphylococcus aureus
Lgia S. Nobre, Vera L. Gonalves and Lgia M. Saraiva

Chapter 12
Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)Hemoglobins
Paul R. Gardner

Chapter 13
Globin Interactions with Lipids and Membranes
Antonio Di Giulio and Alessandra Bonamore

Chapter 14
Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in Escherichia coli
Pauli T. Kallio, Christian J. T. Bollinger, Taija Koskenkorva, and Alexander D. Frey

Chapter 15
Applications of the Vitreoscilla hemoglobin (VHb) gene (vgb) for improved microbial fermentation processes
Xiao-Xing WEI, Guo-Qiang CHEN

Chapter 16
Expression and Purification of Cgb and Ctb, the NO-Inducible Globins of the Foodborne Bacterial Pathogen Campylobacter jejuni
James L. Pickford, Laura Wainwright, Guanghui Wu, Robert K. Poole

Chapter 17
Mapping Heme-Ligand Tunnel in Group I Truncated(2/2) Hemoglobins
Alessandra Pesce, Mario Milani, Marco Nardini and Martino Bolognesi

Chapter 18
Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobins
Paolo Ascenzi and Paolo Visca

Section III. Other Hemoglobins

Chapter 19
Expression, purification and crystallisation of neuro-and cytoglobin
Sylvia Dewilde, Kirsten Mees, Laurent Coger, Christophe Lechauve, Michael C. Marden, Alexandra Pesce, Martino Bolognesi, Luc Monees

Chapter 20
Me...