Globins and Other Nitric Oxide-Reactive Proteins, Part B

Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years' experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.

Section I. Nitric Oxide-Metabolising and Detoxifying Enzymes

Chapter 1
Structural Studies on Flavodiiron Proteins
Joo B. Vicente, Maria Armnia Carrondo, Miguel Teixeira and Carlos Frazo

Chapter 2
Biochemical, Spectroscopic and Thermodynamic Properties of Flavodiiron Proteins
Joo B. Vicente, Marta C. Justino, Vera L. Gonalves, Lgia M. Saraiva and Miguel Teixeira

Chapter 3
Kinetic Characterization of the Escherichia coli Nitric Oxide Reductase Flavorubredoxin
Joo B. Vicente, Francesca M. Scandurra, Elena Forte, Maurizio Brunori, Paolo Sarti, Miguel Teixeira and Alessandro Giuffr

Chapter 4
Escherichia coli cytochrome c-nitrite reductase NrfA
Thomas A. Clarke, Paul C. Mills, Susie R. Poock, Julea N. Butt1, Myles R. Cheesman, Jeffrey A. Cole, Jay C. D. Hinton, Andrew M. Hemmings, Gemma Kemp, Christopher Sderberg, Stephen Spiro, Jessica Van Wonderen, David J. Richardson

Chapter 5
The respiratory NO reductase (NorBC) from Paracoccus denitrificans
Sarah J. Field, Faye H. Thorndycroft, Andrey D. Matorin,
David J. Richardson and Nicholas J. Watmough

Chapter 6
Redox-controlled dinitrosyl formation at the diiron-oxo center of NorA
Rainer Cramm, Katja Strube

Chapter 7
Purification and functional analysis of fungal nitric oxide reductase cytochrome P450nor
Li Zhangand Hirofumi Shoun

Chapter 8
A Quantitative approach to nitric oxide inhibition of terminal oxidases of the respiratory chain
Maria G. Mason, Rebecca S. Holladay, Peter Nicholls, Mark Shepherd and Chris E. Cooper

Section II. Sensor Proteins
Chapter 9
Cloning, Expression, and Purification of the N-terminal Heme Binding Domain of the Globin-Coupled Sensors
Jennifer A. Saito, Tracey Allen K. Freitas, and Maqsudul Alam

Chapter 10
Oxygen-Sensing Histidine-Protein Kinases: Assays of Ligand-Binding and Turnover of Response-Regulator Substrates
Marie-Alda Gilles-Gonzalez, Gonzalo Gonzalez, Eduardo H. S. Sousa, and Jason Tuckerman

Chapter 11
Reactions of nitric oxide and oxygen with FNR (regulator of Fumarate and Nitrate Reduction), a global transcriptional regulator, during anaerobic growth of Escherichia coli
Jason C. Crack, Nick E. Le Brun, Andrew J. Thomson, Jeffrey Green and Adrian J. Jervis

Chapter 12
Genome wide identification of binding sites for the nitric oxide sensitive transcriptional regulator NsrR.
Sam Efromovich, David Grainger, Diane Bodenmiller, and Stephen Spiro.

Chapter 13
Methods in Enzymology: Globins and other NO-reactive proteins. Characterization of the NO-reactive transcriptional activator NorR.
Benot D'Autraux, Nick Tucker, Stephen Spiro and Ray Dixon

Section III. Advanced Spectroscopic Methods

Chapter 14
Hemoglobins from Mycobacterium tuberculosis and Campylobacter jejuni: A Comparative Study with Resonance Raman Spectroscopy
Changyuan Lu, Tsuyoshi Egawa, Masahiro M...