CRISPR-Cas Methods

1. A Complete Methodology for the Instruction of CRISPR-based Gene Editing Using a Simplified Cell-free Extract System with Genetic Readout in Bacteria Kristen M. Pisarcik, Brett M. Sansbury and Eric B. Kmiec 2. In silico Analysis of gRNA Secondary Structure to Predict Its Efficacy for Plant Genome Editing Md Mahmudul Hassan, Abul Kashem Chowdhury and Tofazzal Islam 3. Efficient Multiplexed CRISPR-Cas12a Genome Editing in Plants Yingxiao Zhang and Yiping Qi 4. CRISPR-dCas9 Based Targeted Manipulation of DNA Methylation in Plants Basudev Ghoshala and Jason Gardinera 5. Rapid Assembly of Multiplex Natural CRISPR Arrays Robert M. Cooper and Jeff Hasty 6. Assembly and Assessment of Prime Editing Systems for Precise Genome Editing in Plants Simon Sretenovic, Changtian Pan, and Yiping Qi 7. Designing, Performing and Analysing CRISPR-Cas9-mediated Genome Editing Experiments in Leguminous Plant Dipak Gayen and Subhasis Karmakar 8. Generation of Knockout and Fragment Deletion Mutants in Soybean by CRISPR-Cas9 Li Chen, Yupeng Cai and Wensheng Hou 9. Targeted Base Editing in Soybean using a CRISPR-Cas9 Cytidine Deaminase Fusion Li Chen, Yupeng Cai and Wensheng Hou 10. Genome Editing in Potato Using a Hairy Root Transformation System Nathaniel M. Butler and Jiming Jiang 11. Efficient CRISPR-Cas9-Mediated Genome Editing in Tomato Viswanathan Satheesh, Jinkai Li and Mingguang Lei 12. CRISPR-Cas9-Mediated Genome Editing in Rice: A Systematic Protocol for Single and Multi-Target Vector Construction Hirendra Nath Barman 13. Generating Clonal Seeds from Hybrid Rice with CRISPR-Cas9 Chaolei Liu, Chun Wang and Kejian Wang 14. CRISPR-Cas12a-based DNA Detection for Fast Pathogen Diagnosis and GMO Test in Plants Yun-Mu Zhang, Yinong Yang and Kabin Xie 15. CRISPR-Cas9-mediated Gene Editing in Human Induced Pluripotent Stem Cells Stijn L.M. in 't Groen, Mike Broeders, and W.W.M. Pim Pijnappel 16. Cell-Type-Specific CRISPR-Cas9 System with miRNAs Moe Hirosawa and Hirohide Saito 17. CRISPR-Cas9 gene Editing in Mammalian Cells Using LentiCRISPRv2/LentiGuide-puro Vectors Zubaida Sa'id Ameen, Ece Cakiroglu, Serif Senturk, Abdullahi Umar Ibrahhim and Mehmet Ozsoz 18. An Approach to Proximity Ligation by T4 RNA Ligase to Screen sRNA that Regulate CRISPR-Cas systems Ping Lin, Qinqin Pu, and Min Wu 19. Genetic Engineering of a Phage-based Delivery System for Endogenous III-A CRISPR-Cas System against Mycobacterium tuberculosis Li Zheng Jiang, Junwei Wei, Nan Peng and Yingjun Li 20. CRISPR-Cas9-Mediated Genome Editing in Escherichia coli Bacteriophages Yibao Chen, Xiangmin Li, Shuang Wang, Ping Qian, Yingjun Li 21. CRISPR-Cas Genome Editing in the Cellulolytic Bacterium Clostridium thermocellum Jiang Sean Stettner and Carrie Eckert 22. Gene Targeting in Caenorhabditis elegans Using a Combination of Multiple sgRNAs and a Homologous Recombination-mediated Repair Jose Luis Tellez-Arreola 23. Design of Repair Templates for CRISPR-Cas9-triggered Homologous Recombination in Caenorhabditis elegans Li Hyun-Min Kim and Xiaojuan Zhang