Mouse Cell Culture

From the reviews: "This book provides a diverse collection of protocols for mouse cell culture in a text comprising 18 chapters. ... This book is recommended for researchers and students who are interested in studying the culture of cells from animal models." (Elvira M. Guerra Shinohara, Brazilian Journal of Pharmaceutical Sciences, October-December, 2010)

1. The Culture of Mouse Embryonic Stem Cells and Formation of Embryoid Bodies Melany Jackson, A. Helen Taylor, Elizabeth A. Jones, and Lesley M. Forrester 2. Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures Alastair S. Garfield 3. Embryonic Skeletal Muscle Micro-Explant Culture and Isolation of Skeletal Muscle Stem Cells Janet Smith and Deborah Merrick 4. The Embryonic Kidney: Isolation, Organ Culture, Immunostaining, and RNA Interference Jamie A. Davies 5. Explant Culture of Mouse Embryonic Whole Lung, Isolated Epithelium or Mesenchyme Under Chemically Defined Conditions as a System to Evaluate the Molecular Mechanism of Branching Morphogenesis and Cellular Differentiation Pierre-Marie del Moral and David Warburton 6. Isolation, Culture, and Characterisation of Mouse Embryonic Oesophagus and Intestine Jonathan M. Quinlan, Wei-Yuan Yu, and David Tosh 7. Isolation and Culture of Embryonic Pancreas and Liver Zoe D. Burke, Wan-Chun Li, Jonathan M.W. Slack, and David Tosh 8. Isolation and Culture of Mouse Satellite Cells Antonio Musaro and Laura Barberi 9. Analysis of Cardiac Myocyte Biology in Transgenic Mice: A Protocol for Preparation of Neonatal Mouse Cardiac Myocyte Cultures Nigel J. Brand, Enrique Lara-Pezzi, Nadia Rosenthal, and Paul J.R. Barton 10. Short and Long-Term Cultivation of Embryonic and Neonatal Murine Keratinocytes Reto Caldelari and Eliane J. Muller 11. Isolation, Culture, and Analysis of Mouse Mammary Epithelial Cells Matthew J. Smalley 12. Isolation and Culture of Mouse Pancreatic Islets for ex vivo Imaging Studies with Trappable or Recombinant Fluorescent Probes Magalie A. Ravier and Guy A. Rutter 13. Isolation and Culture of Adult Mouse Hepatocytes Wan-ChunLi, Kate L. Ralphs, and David Tosh 14. Isolation and Culture of Mouse Intestinal Cells F. Charles Campbell 15. Derivation of Primary Choroid Plexus Epithelial Cells from the Mouse Trevelyan R. Menheniott, Marika Charalambous, and Andrew Ward 16. The Preparation of Primary Cortical Neuron Cultures and a Practical Application Using Immunofluorescent Cytochemistry Carla Sciarretta and Liliana Minichiello 17. Cell Culture of Primary Cerebellar Granule Cells Dana Kramer and Liliana Minichiello 18. Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain Henrik Ahlenius and Zaal Kokaia